Double immunogold staining method for the simultaneous ultrastructural localization of regulatory peptides.
نویسندگان
چکیده
منابع مشابه
Ultrastructural localization of alpha-actinin and filamin in cultured cells with the immunogold staining (IGS) method
Monospecific antibodies to chicken gizzard actin, alpha-actinin, and filamin have been used to localize these proteins at the ultrastructural level: secondary cultures of 14-d-old chicken embryo lung epithelial cells and chicken heart fibroblasts were briefly lysed with either a 0.5% Triton X-100/0.25% glutaraldehyde mixture, or 0.1% Triton X-100, fixed with 0.5% glutaraldehyde, and further per...
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The progesterone receptor has been localized in the rabbit uterus by immunocytochemistry at the electron microscopic level, using monoclonal antibodies and the protein A-gold technique. The progesterone receptor in uterine stromal cells was mainly localized in the nucleus; however, a small fraction of antigen was present in the cytoplasm, where it was associated with the rough endoplasmic retic...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1983
ISSN: 0022-1554,1551-5044
DOI: 10.1177/31.7.6189888